Shaking table model test is one of the important methods to study the mechanism of earthquake damage and evaluate the anti-seismic capacity of the overall structure, how to deal with the similitude relation between test model and prototype and how to deduce the response of prototype from the response of model correctly are the key issues. A model of twelve-storey high-rise building frame structure was designed and tested based on the shaking table test, and dynamical analysis under seismic action for prototype and model is done by large nonlinear finite element analysis software MSC.Marc. The seismic response of prototype structure is deduced from model response according to the dynamic similarity relation, and compared with the calculated value of prototype structure. The accuracy of similitude relationship between model and prototype structure is verified by comparing the natural vibration properties, acceleration, displacement and shear, vibration table model test could reflect truly the seismic response of prototype structure, thus the earthquake resistant behavior of prototype structure could be evaluated according to the test results of model.