Ride comfort evaluation of high-speed trains has vital importance for continuously improving the structural design and passengers’ ride satisfaction. This paper tries to evaluate the long-time and momentary ride comfort of high-speed trains in China in the light of the European standard EN12299-2009, and discuss the applicability of the standard. Firstly, objective vibration test and subjective comfort evaluation were carried out in a comprehensive inspection train. Secondly, mean comfort index and discrete events index recommended by the EN12299-2009 standard were briefly introduced, and the two indexes were selected for long-time comfort evaluation and momentary comfort evaluation respectively. Then for the three kinds of track sections with different subjective feelings, based on the measured vibration acceleration data, the long-time and momentary comfort evaluation indexes were calculated respectively, then the comfort of high-speed train and track quality were analyzed. Finally, combined with the subjective evaluation results, the applicability of the corresponding indexes was analyzed, and attempts were made to put forward suggestions for improvement. according to which the comfort of high-speed rail ride and the quality of the track into the standard is analyzed, Finally, the applicability of these indices was analyzed by referring to the subjective evaluation, and certain suggestion was proposed to modify the indexes.