In this paper，a translational-rocking coupling dynamic analysis model of three-dimensional seismic isolation structure is established and its dynamic equation is given. The theoretical expression of the rocking response of the isolation layer is obtained.The correlation analysis of parameters such as horizontal，vertical，rocking frequency ratio and damping ratio of the isolation layer is carried out. It is found that the rocking response has a peak interval with the increase of the horizontal，vertical，and rocking frequency ratios，but generally shows a decreasing trend，and the excitation frequency of the rocking motion is controlled by the input seismic frequency and horizontal motion frequency. The shaking table test of the three-dimensional seismic isolation model is conducted，and the law of the rocking response changing with the vertical frequency ratio is compared and verified. The seismic response analysis of the structure with different aspect ratios is carried out. It is found that the rocking frequency of the three-dimensional seismic isolation structure is close to the rocking excitation frequency and causes resonance. The rocking response has a peak interval with the change of the aspect ratio，but the overall trend is increasing.