In order to accurately measure the vibration acceleration of the structure and provide a scientific and efficient judgment for whether the vibration causes discomfort to the user and affects the normal use of the structure， an intelligent inertia evaluation system is designed by using IEPE piezoelectric sensor and microcomputer Raspberry Pi based on Python. The evaluation standards of human-induced vibration comfort of structures in China， the United States and Europe are all written into the system， which can minimize the cost and size This evaluation system can realize the functions of wireless transmission， data processing and vibration comfort evaluation rapidly. Based on the above functional objectives， taking the laboratory footbridge as a research object， com? pared with the acceleration response collected by the dynamic test and analysis system of DASP engineering version， the relative error of the main frequency of the amplitude spectrum is between 0.02% and 2.85%， which verifies the acquisition accuracy of the sensor system. Taking the pedestrian footbridge and the floor of the teaching building，residential building and dormitory as the re? search object to test the vibration comfort under human-induced excitation. The results show that the intelligent inertial sensing sys? tem can measure the acceleration response of the structure efficiently and in real time， and it can judge the vibration comfort accu? rately， which provides reliable evaluation results of vibration comfort for researchers.