In this paper， a hybrid experimental simulation method of isolation structure based on GPU parallel computing， reduced degree of freedom and considering nonlinear coupling mechanical properties of isolation bearings is proposed to accurately evaluate the real response characteristics of coupling between rubber isolation bearings and superstructure under seismic action. The restoring force performance of each floor of the superstructure is analyzed by numerical simulation， and the superstructure is simplified into a nonlinear MDOF shear model. Combined with the pseudo-static test of the isolation bearing， the isolation bearing model considering nonlinear coupling is established， and the reduced degree of freedom （DOF） substructure coupling mixed experimental numerical model of the structure is obtained. Through the dynamic test of the isolation bearing and the numerical interaction calculation of the substructure， the mixed simulation test of the RC isolation frame under different earthquake actions is carried out. The test results show that in the mixed test of 10-story RC isolation structure， the deformation of isolation layer is in good agreement with the numerical simulation results. The calculation results are in good agreement with numerical simulation， and the hybrid test method is used to analyze the structure of degree of freedom to shrink， which can better restore the upper structure dynamic characteristics and get the rubber isolation bearing the real response under seismic action， and to ensure the good analytical speed and precision.