In view of the shortcomings of traditional vibration testing instruments in portability and real-time performance， a realtime monitoring module is developed based on smart phones， and the accurate acquisition of structural vibration signals and comfort analysis are realized. The accuracy and performance of the testing vibration signal from smart phones are verified by structural shaking table test and floor vibration test. In order to overcome the shortcomings of the traditional comfort evaluation methods in the scope of application and the types of evaluation parameters， the idea of using the fourth power vibration dose value of displacement and speed as the comfort evaluation index is proposed， and the comprehensive comfort limit requirements for bridges and structures are given. Smart phones are used to test the vertical vibration of one footbridge and one building floor， and the comfort is systematically and deeply evaluated through multiple indexes and the limitations. The results show that for the comfort evaluation of long flexible structure， the fourth power vibration dose value of displacement and velocity should be taken as the supplementary evaluation index， and the multi segment data should be comprehensively analyzed.