In order to accurately analyze the influence of temperature and shear deformation effects on the natural vibration charac? teristics of the improved composite box girder with corrugated steel webs， a method for analyzing the natural vibration characteris? tics of an improved composite box girder with corrugated steel webs considering temperature and shear deformation effects is pro? posed in this paper. Considering the influence of the temperature， shear deformation and stiffness correction of the corrugated steel web， the analytical formula of the natural frequency of the improved composite box girder with corrugated steel webs is deduced by using the principle of stress equivalence； The correctness of the analytical formula of natural frequency is verified by using the AN? SYS finite element results and the measured results of bridge； The effects of temperature equivalent axial eccentric force， variation of elastic modulus， shear deformation， and temperature effect under different height-span ratios and different width-span ratios on the natural frequencies of bridge are analyzed. The results show that the fundamental frequency of the improved composite box gird? er with corrugated steel webs is greatly affected by the temperature effect， and the influence of the temperature effect needs to be considered when calculating the fundamental frequency of this bridge type； The shear deformation effect of the corrugated steel web has a significant influence on the natural frequencies of the bridge type， and the influence of shear deformation from the 4th or? der natural frequencies has exceeded 50%； Under different height-span ratios， the temperature effect has a greater influence on the fundamental frequencies， and it increases linearly and sharply with the increase of the height-span ratios； Under different widthspan ratios， the temperature effect has little influence on the natural frequencies and can be ignored. The research results can pro? vide a reference for the calculation and analysis of the natural frequencies of the improved composite box girder with corrugated steel webs.