Tianjin Metro Line 6 is adjacent to Beiyangyuan Campus of Tianjin University， and the train operation may cause adverse vibration effects on important infrastructures of the campus. In this paper， the National Facility for Earthquake Engineering Simulation of Tianjin University， which is close to the line， is taken as the research object. Firstly， the site vibration test is carried out on the sites distributed along the line and the adjacent constructions， through which the vibration attenuation law along the site and the vibration level of the control points in the constructions are studied. Furthermore， a whole process finite element model of tunneling-site-facility constructions is established， and the vibration simulation analysis of site and facility constructions is carried out considering whether the protected river exists or not （actually there is a protected river）. The vibration isolation efficiency of the protected river is emphatically discussed. The research shows that the vibration impact of the operation of Tianjin Metro Line 6 on the large-scale earthquake engineering simulation research facilities of Tianjin University meets the limit requirements of the code. Affected by Tianjin soft soil and the protected river， the vertical acceleration attenuation of the site is significant， and the attenuation rate reaches 97.33% from 0m to 80 m. The design of the mass concrete foundation of the facility and its bottom pile foundation is helpful to the vibration control of the foundation itself. Due to the presence of protected river， the amplitude of the site and the large shaking table foundations was reduced by 40.87% and 27.97%， and the frequency spectrum of the site showed obvious “double peak” phenomenon in 0~20 Hz and 40~80 Hz bands.